The Department of Biomedical Engineering is honored to host a seminar at 2 p.m. Friday, Nov. 13 via Zoom by Dr. Vince Rossi, associate professor in the Department of Physics at Utah Valley University.
Zoom meeting ID: 913 1323 5761. Password: 492839.
Seminar abstract: “Cytometry (FC) was widely adopted across biological and medical laboratories by the 1980’s. In its first incarnation, FC was a scatter imaging system, using optically integrated Side (SSC) versus Forward Scattering Channels (FSC) to generate 2D heat maps to differentiate between cell populations. Improved differentiation between sample populations has grown since with the introduction of fluorescence channels into FC and via Imaging Flow Cytometry (IFC). Fourier Goniometric Flow Cytometry (FGFC) returns to the scattering fundamentals on which FC was developed. Instead of optically integrating FSC and SSC channels to single photodiodes, FGFC maps samples’ scatter functions to the Fourier plane, thereby maintaining angular resolution. The added angular resolutions serves as an additional parameter space that can be used to differentiate between sample populations. This presentation will cover the theoretical modeling and algorithm developed for improved differentiation between sample populations via FGFC over traditional scatter-based FC. FGFC system design will be covered, as well as the method of calibrating the angle space in the Fourier plane of the system. Proof-of-principle imaging of polystyrene microspheres will be included along with future directions for this work.”